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  • Pierce™ His Protein Interaction Pull-Down Kit - Thermo Fisher Scientific
    Thermo Fisher Scientific Pierce His Tag Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins
  • His Tag Pulldown Assay Protocol - University of San Diego
    • Express, purify and dialyze prey protein in pull-down assay buffer • If prey protein is His-tagged, remove using appropriate protease tag (TEV or Thrombin) and remove un-digested protein and His-tag fragments using the TEV protease protocol • Determine protein concentration
  • Dynabeads His-Tag Isolation Pulldown - Thermo Fisher Scientific
    Dynabeads™ His-Tag Isolation Pulldown contains 40 mg beads mL in 20% ethanol and has a capacity of isolating 40 μg of a 28 kDa histidine-tagged protein mg (25 μL) beads Product description Dynabeads™ His-tag Isolation Pulldown is used for the isolation of histidine-tagged proteins The optimized cobalt-based immobilized metal affinity
  • 6X His Protein Pulldowns: An Alternative to GST
    Among fusion tags, His-tag is the most widely used and has several advantages including: 1) It’s small in size, which renders it less immunogenically active, and often it does not need to be removed from the purified protein for downstream applications; 2) There are a large number of commercial vectors available for expressing His-tagged
  • Thermo Scientific Pierce His Protein Interaction Pull-Down Kit . . .
    The PolyHis Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a histidine (6xHis) fusion tag
  • Smart-His™ His Pull-Down Kit - engibody. com
    His pull down is the fusion of a known protein with his epitope, which binds affinity to Ni magnetic beads through a his tag Simply put, we assume that there may be interaction between X protein and Y protein
  • Pull-Down Assays | Thermo Fisher Scientific - US
    Pull-down assays are useful for both confirming the existence of a protein–protein interaction predicted by other research techniques (e g , co-immunoprecipitation) and as an initial screening assay for identifying previously unknown protein–protein interactions
  • Pierce Pull-Down PolyHis Protein:Protein Interaction Kit
    basic pull-down assay is an in vitro technique that consists of a polyhistidine-tagged bait protein (the researcher’s protein of interest) that can be used to identify putative binding partner(s) (the prey)
  • Dynabeads™ His-Tag Isolation and Pulldown - Thermo Fisher Scientific
    Dynabeads His-Tag Isolation and Pulldown beads are 1-m monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets
  • His-tagged Proteins–Production and Purification | Thermo Fisher . . .
    Protein interaction pull-down—nickel agarose resin can be used to purify, identify and measure interactors of His-tagged proteins; Gel staining—a metal-based fluorescent stain enables detection of His-tagged proteins in SDS-PAGE





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