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  • saline replacement in serum discrepancy - PathLabTalk
    If the rouleaux is such that the initial testing looks "rough" macroscopically upon resuspension, I then look at it microscopically to be confirm that it is rouleaux So after performing the saline replacement, I again look both macroscopically and microscopically because I want to ensure the saline replacement really did get rid of the problem
  • Rouleaux interference - Transfusion Services - PathLabTalk
    We recently had a positive antibody screen in gel, we ran a panel ( also in gel) and got some reactions but no pattern and everything was ruled out We then did an antibody screen in tube and saw that there was rouleaux at immediate spin but the screen was negative at AHG Is it common to have ro
  • can you tell if Rouleaux is present just from looking at the gel card . . .
    Rouleaux shows up in gel as either haze or mixed-cell (see the pictures) Haze = rouleaux (there's nothing else significant that causes this picture) Period Yes, in tube testing it's very hard to tell rouleaux from weak agglutination unless you use the microscope and or saline replacement This is not the case in gel Leave it, you are done
  • Is the full crossmatch valid after saline replacement
    We have a patient with strong rouleaux and an alloantibody According to our policy, we need to perform a full crossmatch which means the crossmatch is carried from immediate spin, to 37C and to AHG phases Due to the strong rouleaux, a saline replacement technique has to be used during the immediate spin crossmatch phase
  • Rouleaux - Transfusion Services - PathLabTalk
    Hello, I currently use Gel as my primary method A tech on the weekend got the following: 3 cell screen: 2+ for allGel panel A (11 cells) 1+ and 2+ reactions with the exception of 2 cells and the Auto control that were neg Gel panel B (11 cells) 1+ and 2+ reactions with the exception of 3 cells t
  • Rouleaux or cold auto - Transfusion Services - PathLabTalk
    EDTA tubes will have more rouleaux because of the higher protein level We also get surprizes, because we use gel, so never see tube reactions until we do IS XMs For patients with negative antibody screens who show 'stickiness' at IS (we also have a cold BB room), we examine for rouleaux, if not, we do a quick (5 minutes) 37C inubation
  • Rouleaux in Ortho Gel Testing - Transfusion Services - PathLabTalk
    We are seeing rouleaux fairly frequently that appears in one screen cell and not the other, and in some of the gel panel cells the same way This is in a Ortho IgG gel card ran on a Vision analyzer The Rouleaux is verified in tube under the scope We normally run a PEG tube screen and panel on t
  • Dealing With Cold Agglutinins - Transfusion Services - PathLabTalk
    As far as the rouleaux not being an issue with the reverse typing, the Echo testing platform cannot reliably detect hemagglutination reactions that are graded as 1+ or less in test tube methodology The IS XM reactions with the example above were graded as W+ by the tech
  • Microscopes in the Blood Bank - PathLabTalk
    If you use plasma instead of serum the increased protein can enhance rouleaux Sometimes it is difficult to differentiate rouleaux from true agglutination under the scope The novice general Med Tech (or the OCD Med Tech) tend to see two or three red cells "holding hands" under the scope and call this a W+micro reaction
  • Student Specimens - Transfusion Services - PathLabTalk
    Kaleigh, whenever we encountered a specimen showing nice rouleaux I would gather all the old lab specs on the patient, pool them and freeze aliquots to torment future generations of students You could also have your buddies in Hematology be on the lookout for them as well





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